Fecha/hora: 01/06/2024 01:38:46

Biblioteca(s): 	INIA La Estanzuela.	
Fecha corriente: 	01/10/2019	
Fecha de la última actualización: 	01/10/2019	
Autoria: 	BÁEZ, F.; CAMARGO, A.A.; GASTAL, G.D.A.	
Afiliación: 	FRANCISCO BÁEZ, University Center of Tacuarembó, UDELAR, Route 5, Km 386, Tacuarembó, Uruguay.; ÁLVARO A. CAMARGO, University Center of Tacuarembó, UDELAR, Route 5, Km 386, Tacuarembó, Uruguay.; GUSTAVO DESIRE ANTUNES GASTAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.	
Título: 	Ultrastructural imaging analysis of the zona pellucida surface in bovine oocytes.	
Año de publicación: 	2019	
Fuente/Imprenta: 	Microscopy and Microanalysis, 1 August 2019, Volume 25, Issue 4, Pages 1032-1036.	
DOI: 	10.1017/S1431927619000692	
Idioma: 	Inglés	
Notas: 	Article history: Received 21 August 2018/ Revised 23 April 2019/Accepted 23 April 2019.	
Contenido: 	Abstract:
The aims of the present study were to: (i) evaluate the ultrastructural differences in the zona pellucida (ZP) surface between immature and
mature bovine oocytes, and (ii) describe a new objective technique to measure the pores in the outer ZP. Intact cumulus oocyte complexes
(COCs) obtained from a local abattoir were immediately fixed (immature group) or submitted to in vitro maturation (IVM) at 38.5 °C for 24 h in a humidified atmosphere of 5% CO 2 in air (mature group). Oocytes from both groups were morphologically evaluated via Scanning Electron Microscopy (SEM) and the images were processed in the Fiji/ImageJ software using a new objective methodology through the Trainable Weka Segmentation plugin. The average number of pores in ZP was greater (p < 0.05) in the mature group than the immature group. However, the size and circularity of pores in ZP did not differ (p > 0.05) between groups. In conclusion, it has been shown that the number of pores highlighted the main ultrastructural change in the morphology of the ZP surface of bovine oocytes during the IVM pro-cess. We have described an objective method that can be used to evaluate ultrastructural modifications of the ZP surface during oocyte maturation and early embryo development.	
Palabras-Clave: 	BOVINE; IN VITRO MATURATION; PLATAFORMA SALUD ANIMAL; ULTRASTRUCTURE; ZONA PELLUCIDA.	
Categoría del tema: 	--	
Registro original: 	INIA La Estanzuela (LE)	
Ejemplares: 	LE - (103017-1) - PXI - AP - DD - PP/Microscopy and Microanalysis/2019 -  -  - DIS